Hypoxia and hypoxia-inducible factor (HIF) downregulate antigen-presenting MHC class I molecules limiting tumor cell recognition by T cells
Fig 4
Hyperoxia upregulates MHC class I expression equally in 2D and 3D cultures.
(A-D): MCA205 tumors were grown as 2D monolayers (A, C) or as 3D spheroids (B, D) at 21% O2 or 60% O2 for 48h. MHC class I levels were determined by flow cytometry. The magnitude of MHC class I upregulation was similar in 2D and 3D cultures. Representative histograms (A, B) and associated quantification and statistics (C, D) of 4 independent experiments shown. The significance of differences was analyzed by the Student’s t-test (two-sided); p = 0.002 (C), p = 0.001 (D). Grey filled: Unstained control; Blue: Normoxia (21% O2); Green: Hyperoxia (60% O2). Error bars indicate SD.