A novel function of twins, B subunit of protein phosphatase 2A, in regulating actin polymerization
Fig 5
Depletion of twins function impairs ovarian border cell migration and cytoplasm dumping.
(A, A’) Stage 10b of wild type GLC ovary showed that border cells (white arrowhead) migrated to the edge of the oocyte. (B, B’) twins mutant GLC ovary appeared to slow border cell migration. Green represents border cells; red shows phalloidin staining. (C) Table shows statistical analysis. p<0.05 by Fisher’s exact test analysis. (D, E) twins mutant nurse cells were incapable of dumping the cytosol into the oocyte and still occupied the anterior space of the egg. Green shows nuclear staining by sytox; red for phalloidin. Insert B&W images show abnormal egg morphology of twins GLC with shorter egg length and malformed appendages compared to the wild type GLC (E). Genotype: (A, D) yw, hsFLP; slbo-GAL4, UAS-GFP; FRT82B tub-β-gal/FRT82B OVOD1. (B, E) yw, hsFLP; slbo-GAL4, UAS-GFP; FRT82B twins60/FRT82B OVOD1. Scale bar, 80 μm (B”, E), 100 μm (E insert).