BRCA1 protein expression and subcellular localization in primary breast cancer: Automated digital microscopy analysis of tissue microarrays
Fig 2
IHC staining and corresponding digital annotation for BRCA1 in a representative core.
A: IHC staining utilizes MS110 mouse monoclonal antibody to assess the level of BRCA1 protein expression. B: Tissue segmentation where the epithelial compartment is pink and the stromal compartment is yellow. C: Nuclear segmentation. D: nuclear score map. Definition of the digital image annotation intensity score: blue = 0, yellow = 1, orange = 2 and red = 3. The attached legend to the right indicates what each color stands for in the tissue segmentation and score map.