Development of a fluorescent reporter system for monitoring ER stress in Chinese hamster ovary cells and its application for therapeutic protein production
Fig 7
Key elements of ER stress pathway responsible for efficient IgG secretion.
Schematic representation of secretion inhibitory and secretion promoting roles regulated by PERK and IRE1 pathway proteins. By inhibiting the PERK pathway, cells with high P58IPK levels signal the activation of the IRE1 pathway and overexpression of protein chaperones (BiP, GRP94 and PDI) necessary to cope with expression levels of high producing cells.