Therapeutic potential of tonsil-derived mesenchymal stem cells in dextran sulfate sodium-induced experimental murine colitis
Fig 2
Immunophenotypes and differentiation capacity of the cell surface of TMSC.
(A) Representative flow cytometry analysis of TMSC are shown. The cells were labeled with antibodies against human antigens, CD14, CD34, CD45, CD73, CD90, and CD105. The shaded histogram indicates isotype control and the open histogram represents positive reactivity with the indicated monoclonal antibody. (B) TMSC were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages for 3 weeks using the appropriate differentiation medium. For a control experiment, TMSC were cultured in the absence of differentiation medium. The level of adipogenesis, osteogenesis, and chondrogenesis was assessed by staining with Oil red O, Alizarin red S, and Alcian blue, respectively. Scale bar: 200 μm.