A role for the tfs3 ICE-encoded type IV secretion system in pro-inflammatory signalling by the Helicobacter pylori Ser/Thr kinase, CtkA
Fig 4
Signalling pathways involved in CtkA-mediated stimulation of IL-8 secretion.
MKN28 cell monolayers remained untreated (-), or were treated with chemical inhibitors for NF-κB (6 amino-4-(4-phenoxyphenylethylamino) quinazoline), JNK (SP600125) and MEK1 (U0126) for 1 h before and during infection with H. pylori strain AB5ΔcagE::gsk-ctkA. IL-8 levels were subsequently assessed by ELISA. Basal levels of IL-8 detected in uninfected supernatants are also indicated for reference (‘Cells’). Graph shows means and SDs from three independent experiments, each performed in triplicate. p values indicate significant differences in the presence of inhibitor compared with infection alone.