Single-cell profiling of dynamic cytokine secretion and the phenotype of immune cells
Fig 4
Bead-based nanowell arrays can enable monitoring the dynamic IFN-γ secretory activity.
(A) Schematic of immuno-sandwich design for detecting IFN-γ secretion from single NK cells using nanowell arrays. (B) Dynamic tracking of the IFN-γ secretory activity of an NK cell within the same nanowell: tSecrete is 90 min. Scale bar = 10 μm. (C) Four representative examples of dynamic fluorescence intensity (MFI) of the beads (IFN-γ secretion) upon activation of individual NK cells. The best-fitting response curve is overlaid on top of the raw data (triangles). The tSecrete (red), Hill slope and MFI ratio are shown for each of the NK cells secreting IFN-γ.