Myeloid-specific deletion of NOX2 prevents the metabolic and neurologic consequences of high fat diet
Fig 2
Effects of HFD on adipose macrophage infiltration, hypertrophy, and injury in NOX2-FL and mNOX2-KO mice.
Visceral epididymal fat pads were collected at the end of the 16-week feeding trail. (A) Blinded quantification of crown-like structures (CLSs) composed of continuous Iba-1-positive macrophages. Data are means and SEM, and *** and * indicates significant (p < 0.001 and p < 0.05) increases in CLS in HFD-fed NOX2-FL and mNOX2-KO mice compared to mice on CD, while ### depicts significant decrease in CLS in HFD-fed mNOX2-KO mice as compared to HFD-fed NOX2-FL mice. (B) Representative images of Iba-1 immunostaining in epididymal fat from all groups of mice. (C) Epididymal adipocyte size. Data are means and SEM, and ** and *** indicates significant (p < 0.01 and p < 0.0501) increases in adipocyte size in HFD-fed NOX2-FL and mNOX2-KO mice compared to mice on CD. # depicts significant decrease in epididymal adipocyte size in CD-fed mNOX2-KO mice as compared to CD-fed NOX2-FL mice. (D) Expression of GADD153/CHOP and GRP78 in tissue homogenates prepared from epididymal adipose depots. Data depict mean ± SEM expression in HFD mice presented as % values in CD mice (100% line on graph). *** indicates significant (p < 0.001) increases in expression in HFD-fed NOX2-FL mice compared to CD-fed NOX2-FL mice, while ### indicates significant (p < 0.001) decreases in expression in HFD-fed mNOX2-KO mice as compared to HFD-fed NOX2-FL mice.