Determination of absolute expression profiles using multiplexed miRNA analysis
Fig 1
Schematic illustration of the Ligo-miR EZ multiplex miRNA assay.
In the capture ligation, a universal adapter is ligated to each miRNA to form a template molecule. In the coding ligation, a miRNA specific discrimination probe and common probe are hybridized to each template and ligated together to form a single stranded Ligo-miR product. Thermal cycling is used to generate up to 50 products from a single template. Finally, the Ligo-miR products are analyzed by denaturing PAGE. Shown are 4 samples analyzed using a 24-plex Ligo-miR EZ probe set. Each band is a specific miRNA product where band intensity is proportional to quantity.