Visual detection of Brucella in bovine biological samples using DNA-activated gold nanoparticles
Fig 3
(a) Photographs of the assay results (after addition of HCl), for the detection of genomic DNA from Brucella at different concentrations (negative control is E.coli genomic DNA at 60 ng/μl) performed in tubes. Total volumes of the reaction mix in each tube is 25 μl. Quantities are indicated for 1 μl of sample before dilution in the reaction mix. (b) UV-Vis spectra of gDNA sensitivity showing the absorbance peak at ~520nm for positive samples and red shifting with absorbance peak at higher wavelength in both negative and control samples, with a limit of detection of 5ng/μl in the sample before dilution in the reaction mixture. S3 Fig. Enhanced photographs of all figures (3–6) are available as supplementary information to provide more apparent color differentiation.