Indole-3-carbinol, a plant nutrient and AhR-Ligand precursor, supports oral tolerance against OVA and improves peanut allergy symptoms in mice
Fig 2
Quantification of I3C and metabolites in mouse serum after oral uptake.
Mice were fed for up to 7 days with chow containing 2 g/kg I3C. For the 15 minute value, mice received 5 mg/I3C per mouse diluted in 10%DMSO/olive oil by gavage. Serum samples were prepared and analyzed for the presence of I3C, DIM, and ICZ. Sera were spiked with 4-methoxyindole as the internal standard to calibrate for possible differences in extraction efficiency. Note that I3C is detectable only after the bolus feeding, but not when I3C was given in the chow, and is thus eaten in smaller amounts over the entire day. Y-axis: arbitrary units.