ECIS technology reveals that monocytes isolated by CD14+ve selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1β activated endothelium in comparison to TNFα
Fig 3
Representative temporal profile of endothelial barrier integrity following the addition of enriched monocytes, reflecting monocyte-endothelial activation.
The endothelial barrier resistance was measured using ECIS Zθ technology. The top panels are under basal conditions, middle panels are IL-1β activated and bottom panels are TNFα activated endothelium, which were activated 24 h prior to monocyte addition. Monocyte addition is demarcated by the dotted vertical line (~52 h). Monocytes isolated using CD14+ve method and the untouched method were added onto the endothelial cells at the effector to target ratio of either 1:1 (red line) or 1:10 (blue line). Black line represents endothelial barrier integrity of cells that received media only (no monocytes). Each line represents the mean ± SEM of 4 wells.