Formation and spreading of TDP-43 aggregates in cultured neuronal and glial cells demonstrated by time-lapse imaging
Fig 7
Adenovirus-transduced artificial TDP-43 aggregates formation in astrocyte and oligodendrocyte leads to cell death.
Time-lapse imaging of adenovirus-transduced artificial TDP-43 cytoplasmic aggregate formation in glial cells. GFAP-positive 1464RGFAPpEGFP astrocytes (a) and CNP-positive 1464RCNPpEGFP oligodendrocytes (b) were transduced with AxDsR-WT.TDP43 and AxDsR-CTF.TDP43 (DsR-(WT+CTF)) (red) in the presence of MG-132. Transduced EGFP-positive astrocytes (green) (a) and oligodendrocytes (green) (b) are traced for 72 hrs, showing cytoplasmic aggregate formation, cell collapse and cell death (arrows). Scale bar = 20 μm.