Three-dimensional cell shapes and arrangements in human sweat glands as revealed by whole-mount immunostaining
Fig 5
Imaging of sweat gland blood vessels and nerve fibers.
(A) Immunofluorescence detection of CD31 and phalloidin in sweat glands. Arrows and arrowheads indicate secretory portions and ducts of sweat gland tubules, respectively. (B) Double immunofluorescence detection of CD31 and LN332 in sweat glands. Arrowheads indicate blood vessels running parallel to sweat gland tubules. (C) Immunofluorescence detection of PGP9.5 in sweat glands. Arrows indicate nerve fibers wrapping around the tubules of sweat glands. Arrowheads indicate ductal tubules. (D) Immunofluorescence detection of PGP9.5 and αSMA in sweat glands. Lower panel shows optical sections of sweat glands. Arrows indicate nerve fibers that enwrap the αSMA-positive secretory portions. The boxed area in the upper panel is shown at higher magnification in the lower panel (A–C). Nuclei (blue) were counterstained with Hoechst 33342. Scale bars: 50 μm.