Molecular hydrogen protects against oxidative stress-induced SH-SY5Y neuroblastoma cell death through the process of mitohormesis
Fig 5
The H2-induced anti-oxidative defense system.
(A) SH-SY5Y cells were stained with an anti-Nrf2 antibody. H2 treatment induced translocation of Nrf2 into the nucleus (arrow heads). The scale bar is 100 μm. (B) Cells were incubated in culture medium containing either Glc or Gal under N2- or H2-mixed gas for 18 h. The transcript levels of genes involved in the anti-oxidative defense system, including CAT, GCLC, GPX1, GSR, HO-1 and SOD2, were quantified by real-time PCR analysis coupled with reverse transcription of total RNA, and expressed relative to those in cells incubated under N2-mixed gas. (C) The expression levels of proteins involved in the anti-oxidative defense system, including γ-GCSc, HO-1, NQO1, Nrf2 and SOD2, were quantified by the intensity of representative immunoblots (n = 5 or 6, each), and expressed relative to those in cells incubated under N2-mixed gas. *P < 0.05, **P < 0.01. ***P < 0.001.