Similar regulatory mechanisms of caveolins and cavins by myocardin family coactivators in arterial and bladder smooth muscle
Fig 3
Induction of caveolins and cavins by MKL1/MRTF-A and myocardin in rat bladder smooth muscle.
Adenoviral transduction of rat bladder smooth muscle cells with MRTF-A or myocardin (MYOCD) increased mRNA levels of caveolins and cavins (A-F, n = 12–18). Western blotting after transduction demonstrated increases of caveolins and cavins at the protein level that largely matched changes at the mRNA level (G). Summarized data from the western blots is shown in panels H-M (n = 4–12). 18S was used as a house-keeping gene for the qRT-PCR and HSP90 as loading control for western blotting.