Transplantation of HGF gene-engineered skeletal myoblasts improve infarction recovery in a rat myocardial ischemia model
Fig 2
Adenovirus-mediated HGF expression in skeletal myoblasts.
(a) GFP expression in myoblasts transfected with Ad-GFP by fluorescence microscopy (original magnification ×400); (b) Immunocytochemistry staining for human HGF in myoblasts transfected with Ad-HGF (immunocytochemistry staining, original magnification ×400). (c) Immunocytochemistry staining in untransfected control myoblasts (immunocytochemistry staining, original magnification ×400). (d) RT-PCR-based assessment of human HGF gene in myoblasts transfected with Ad-HGF. (e) The RT-PCR analysis of HGF mRNA in SM infected with Ad-HGF, Ad-GFP, and SM only. (f) The Ad-HGF-transduced myoblasts were cultured for 14 days. The conditioned medium was collected at different points in time, and the HGF protein was determined by ELISA. Significant differences were found in myoblasts infected with Ad-HGF (SM-HGF group) as compared to myoblasts infected with Ad-GFP (SM-GFP group) and non-infected myoblasts (SM group) (n = 6, P<0.05, *P<0.05 vs. SM group control cell; #P<0.05 vs. SM-GFP group).