Universal vaccine against respiratory syncytial virus A and B subtypes
Fig 1
Expression and purification of recombinant GcfAB protein.
Schematic diagram of the GcfAB recombinant protein. (A) A pET-21d plasmid containing both a.a 131 to 230 of RSV A2 G protein (GcfA) and a.a. 131 to 230 of RSV B1 G protein (GcfB) was constructed. (B) The GcfAB protein was purified by His-tag affinity chromatography (lane 1) and size-exclusion chromatography (Sephacryl S-200 HR) with (lane 2) / without (lane 3) dithiothreitol (DTT). Purification of GcfAB was confirmed by SDS-PAGE at each purification step, as indicated by the arrow.