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Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions

Fig 4

Carbohydrate content in PpDof5 transgenic poplar.

(A) Analysis of carbohydrate content in 10-week-old control and transgenic poplar lines grown in the growth-chamber. Values are expressed as nmol mg-1 of fresh weight (FW). The determination is the mean of three independent biological samples measured in triplicate in upper leaves (UL), basal leaves (BL), stems and roots. Asterisks indicate that the difference between the control and transgenic poplars is significant by the t-test (P<0.05). (B) Analysis of carbohydrate content in control and transgenic poplar lines grown in the field during the two-growing seasons of the experiment. Values are expressed as nmol mg-1 FW and are the means of three independent biological samples measured in triplicate. The samples were upper leaves (UL) and basal leaves (BL) for 2012 and leaves and branches for 2013. Asterisks indicate that the difference between the control and transgenic poplars is significant by the t-test (P<0.05) for starch content in samples of 2012.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0174748.g004