Transcription factor-induced activation of cardiac gene expression in human c-kit+ cardiac progenitor cells
Fig 1
Lentivirus-mediated delivery of transcription factors to CPCs.
A, Untranduced or mCherry virus-transduced CPCs were imaged at 4 days post-transduction. Fluorescence of mCherry protein is visible in red. DAPI staining of nuclei was pseudo-colored in green. B, Cells transduced with virus expressing mCherry (control), 3xFLAG-tagged Gata4, MEF2C, TBX5 or BAF60C, or V5-tagged NKX2.5 were assayed by Western blot using antibody against each TF. C, Cells transduced with virus expressing 3xFLAG-tagged Gata4, MEF2C, TBX5 or BAF60C, or V5-tagged NKX2.5 were stained for the indicated epitope (i.e., FLAG or V5) which is shown in monochrome. DAPI images are shown in lower panels. Note that exogenous, epitope-tagged transcription factors localize to the nucleus as expected.