Generation of murine tumor cell lines deficient in MHC molecule surface expression using the CRISPR/Cas9 system
Fig 5
Stable β2m KO clones show enhanced susceptibility to NK cell recognition.
Target cells (2.5 x 105) were incubated with (5 x 104) IL2-activated (1,700 IU/ml IL2, 7 d) NK cells for 8 h and IFNγ released by the NK cells was quantified by ELISA. B16F10/M1KO and EO-NY/M1KO clones induce enhanced IFNγ release compared to parental cells or control transfectant clones, respectively. Error bars represent SEM of technical triplicates.