The DRF motif of CXCR6 as chemokine receptor adaptation to adhesion
Fig 6
The DRF motif impairs chemotaxis.
THP-1 cells were transduced with lentivirus encoding murine CX3CR1 variants or EV control. A: Ligand binding was analyzed by incubation with human CX3CL1-Fc fusion protein and FACS analysis (n = 3). B/C: Chemotaxis against increasing concentrations of soluble CX3CL1 was analyzed in a Boyden chamber assay. In B, wild type cells were investigated for chemotaxis towards soluble murine or human CX3CL1 (n = 3), and in C cells overexpressing murine receptor variants were assayed for chemotaxis towards murine CX3CL1 (n ≥ 3). Statistical differences were analyzed by one-sample t-test (B, hypothetical value 1) or Student’s t-test (A-C). Asterisks indicate differences to control (EV in A, random migration in B/C), hashes indicate differences between receptor variants (*/#p<0.05, **/##p<0.01, ***p<0.001).