PnLRR-RLK27, a novel leucine-rich repeats receptor-like protein kinase from the Antarctic moss Pohlia nutans, positively regulates salinity and oxidation-stress tolerance
Fig 4
PnLRR-RLK27 contributes to the salt tolerance in transgenic Physcomitrella patens and Arabidopsis.
(A) RT-PCR analysis revealed that the PnLRR-RLK27 was successfully transcribed in Physcomitrella patens. (B) The size of transgenic Physcomitrella patens gametophyte plants was significantly larger than that of the wild type under salt stress conditions (4-week-old plants). (C) Statistical analysis of gametophyte size as shown in (B). (D) RT-PCR analysis revealed that the PnLRR-RLK27 was successfully transcribed in Arabidopsis. Tubulin gene was used as internal references. (E) The seed germination rate of transgenic seedlings was significantly higher than that of the wild type under salt stress conditions (6 days' seed germination). (F) Statistical analysis of seed germination rates as shown in (E). Seed germination rates were calculated by counting the proportion of the WT plants and the transgenic seedlings bearing open green cotyledons. (G) PnLRR-RLK27 promotes the growth of Arabidopsis seedling after salinity treatment. (H) Measurement of root length of salinity-stressed Arabidopsis seedlings shown in (G). Vertical bars are presented as means ±SE, and asterisks (*) indicate significant differences of means between the transgenic lines and the WT plants at P<0.05. Bar 10 mm.