Application of an optimized flow cytometry-based quantification of Platelet Activation (PACT): Monitoring platelet activation in platelet concentrates
Fig 1
Effect of 10-day storage on platelet activation, measured by light transmission aggregometry (LTA).
Aggregometry was measured in response to 10 μmol/L adenosine diphosphate (ADP, panel A), 4 μg/ml collagen (panel B) and 30 μmol/L thrombin receptor-activating peptide (TRAP-14, panel C). Light transmission in platelet concentrates (PCs) compared to autologous platelet poor plasma is expressed as percentage of maximal aggregation. Data are expressed as mean±SD and a dashed line was set on mean day 2—2SD. * = P<0.01, ** = P<0.001 compared to day 2.