Alpha-Melanocyte Stimulating Hormone Protects against Cytokine-Induced Barrier Damage in Caco-2 Intestinal Epithelial Monolayers
Fig 3
The effect of α-MSH treatment on the permeability of cytokine treated Caco-2 monolayers.
Caco-2 monolayers grown on culture inserts were treated with cytokines (CK; 50 ng/ml TNF-α and 25 ng/ml IL-1β) without or with α-MSH (10−8 M) for 24 hours, then permeability was measured for fluorescein (A) and albumin (B). The control group (C) received culture medium. Means ± S.D., n = 4–6; ***P < 0.001, #P < 0.05. Papp, apparent permeability coefficient. *: CK, CK+MSH compared to C; #: CK+MSH compared to CK, ns: no significant difference to C or CK.