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A New Israeli Tobamovirus Isolate Infects Tomato Plants Harboring Tm-22 Resistance Genes

Fig 2

Morphological and serological characterization of viral particles and coat protein.

(A) Electron micrograph illustration of viral particles. (B) Distribution of viral particle lengths as imaged by TEM showing average size of 235±123nm. (C) SDS-PAGE (15%) analysis of viral particles preparation followed by Coomassie brilliant blue staining depicting the CP at molecular mass of ~17.5 kDa. (D-E) Testing the specificity and the cross reactivity of the antisera raised against the new tobamovirus isolate. By Coomassie brilliant blue staining (D) in parallel to Western blot analysis (E). Purified virions (1), CP from infected tomato plants extract, cv. Ikram (2). An extract from healthy (non-infected) tomato leaves (3). Cross reactivity of the antisera with (4) extracted pepper leaves, cv. Maor infected with PMMoV, (5) extracted Nicotiana tabacum cv. Samsun infected with TMGMV and (6) extracted tobacco leaves cv. Samsun infected with TMV.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0170429.g002