Sensitization of TRPA1 by Protein Kinase A
Fig 1
Activation of PKA by FSK sensitizes TRPA1.
A Calcium-imaging experiments on hTRPA1-transfected HEK293t cells. TRPA1 was activated with six applications of carvacrol (filled bars; 50 μM; 20 s). Top: control experiment without application of FSK. Bottom: activation of PKA by application of FSK (open bar, 10 μM, 120 s) causes strong sensitization. B To allow the full development of sensitization, the sensitization ratio was calculated as the amplitude of the sixth over the fourth response. Sensitization ratio 1.04 ± 0.02 in control (white) and 1.29 ± 0.09 with FSK (black; p = 0.002, one-way ANOVA with Bonferroni post-hoc analysis). Number of cells under each condition is indicated. ** p < 0.01