Regulation of PI-2b Pilus Expression in Hypervirulent Streptococcus agalactiae ST-17 BM110
Fig 3
Role of the PI-2b hairpin-like sequence as a transcriptional terminator.
Detection of a read-through transcript (arrowhead) encompassing the last gene of AgB operon and the first gene of PI-2b locus in strain A909 but not in strain BM110. RT-PCR was performed using the Gly3/Gly4 (lanes 1, 4, 7, 10, 13, 16), ORF1/ORF2 (lanes 3, 6, 9, 12, 15, 18) and Gly1/ORF2 (lanes 2, 5, 8, 11, 14, 17) oligonucleotides pairs (S2 Table). Lanes 1 to 3 and 10 to 12, positive controls using chromosomal DNA (chr); lanes 4 to 6 and 13 to 15, read-through transcript of san1522 (according to COH1 annotation) and orf (cDNA); Lanes 7 to 9 and 16 to 18 represent negative controls without reverse transcriptase (NRT).