TRPC4α and TRPC4β Similarly Affect Neonatal Cardiomyocyte Survival during Chronic GPCR Stimulation
Fig 2
Spontaneous Ca2+ influx in TRPC4α and TRPC4β overexpressing neonatal rat cardiomyocytes.
A, Representative Fura-2 Ca2+ recordings of neonatal rat cardiomyocytes (NRCs) infected with either Ad-βgal, Ad-TRPC4α or Ad-TRPC4β. Cells were perfused with a nominally Ca2+-free Tyrode solution including verapamil (10 μM). After 50 s, Ca2+ influx was challenged by perfusing the cells with a solution containing 1.8 mM Ca2+. B, Mean Δ ratio values were calculated by subtracting the peak value at 1.8 mM from the baseline value at 0 mM Ca2+.*P<0.05 vs Ad-βgal. Cells from three different experiments were analyzed. N-number of cells is indicated in the bars.