Ginkgolide B Exerts Cardioprotective Properties against Doxorubicin-Induced Cardiotoxicity by Regulating Reactive Oxygen Species, Akt and Calcium Signaling Pathways In Vitro and In Vivo
Fig 6
GB exert its protective effect by activating the PI3K/Akt pathway and inhibiting the CaMKII pathway.
Cardiomyocytes cell viability analysis was determined by MTT assay. PI3K inhibitor LY294002 (10 μM) pretreatment inhibited the protective effect of GB pretreatment in DOX induced cardiotoxicity while CaMKII inhibitor KN62 (10 μM) pretreatment significantly increased cell viability. ****p<0.0001 when compared to control group, #p<0.01 when compared to DOX group.