A Broad-Spectrum Chemokine-Binding Protein of Bovine Papular Stomatitis Virus Inhibits Neutrophil and Monocyte Infiltration in Inflammatory and Wound Models of Mouse Skin
Fig 4
Myeloperoxidase analysis revealed that CBP blocks neutrophil infiltration in a murine skin inflammatory model.
Twelve mice were divided into 3 groups of 4 and injected in the dermis with 1 μg LPS with and without the indicated amounts of CBP. In addition each mouse received injections of CBP only and PBS. i.e. a total of four injections per mouse. Each group received a different dose of CBP as shown above. At 12 h post-injection, mice were euthanized and 6 mm skin samples were taken and homogenized in HTAB to extract MPO. The harvested solution was added to TMB substrate in duplicate, and the enzyme activity was measured spectrophotometrically at 450 nm. The mean ± SD of the combined data of two independent experiments are shown and the asterisk indicates a significant difference (P < 0.05, Tukey’s test ANOVA, GraphPad Prism).