A2A Adenosine Receptor Antagonism Reverts the Blood-Brain Barrier Dysfunction Induced by Sleep Restriction
Fig 5
A2A adenosine receptor antagonism attenuates Iba-1 overexpression induced by sleep restriction.
Representative western blot images of expression of Iba-1 per region (cortex, hippocampus, basal nuclei, and vermis) are shown, as well as graphs depicting the relative optical density of Iba-1 in the following groups: control plus DMSO (Con), sleep restriction plus DMSO (SR), and sleep restriction plus SCH58261 at 0.1mg/kg (SR+SCH) (n = 3 per group). GAPDH was used for normalization. Mean ± s.e.m. Two-way ANOVA test, post hoc orthogonal contrast codes, *p<0.05 as compared to the control group; #p<0.05 with respect to the sleep restriction group. In the lower panel, microphotographs show the differences in the number of Iba-1 immunoreactive cells in the basal nuclei of control, sleep restricted and sleep restricted plus SCH groups. X400.