Antigenicity of a Bacterially Expressed Triple Chimeric Antigen of Plasmodium falciparum AARP, MSP-311 and MSP-119: PfAMSP-Fu35
Fig 6
Inhibition of parasite invasion in vitro by antibodies specific to AMSP-Fu35 and specificity of the inhibition observed.
(A) IgGs purified from rabbit sera immunized with AMSP-Fu35 formulated with different adjuvants were tested at 1mg/ml, 2.5mg/ml and 5mg/ml for inhibition of erythrocyte invasion in P. falciparum 3D7. Percent growth inhibition (shown as means ± standard deviations) was calculated by using the parasitemia in the presence of immune sera with respect to parasitemia in the presence of pre-immune sera. (B) Purified IgGs against AMSP-Fu35 was tested for its invasion inhibitory specificity against the 3D7 parasite clone. For studying the invasion inhibitory specificity, total IgGs were pre-incubated with recombinant proteins AMSP-Fu35, PfMSP-119, PfMSP-311 and PfAARP, at the final concentrations of 25μg/ml, 50μg/ml and 100μg/ml. The white bar denotes the invasion inhibitory activity of AMSP-Fu35 IgGs.