Endocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells
Fig 3
Effects of knocking down selected genes on ANDV replication.
Western blots showing ANDV glycoprotein (Gc) and nucleoprotein (N) in HMVEC-L transfected with gene-specific siRNAs against (A) DNM2 or CLTC; (B) AP2M1, CAV1, or CDC42; (C) ARF6; (D) NSF; (E) RAB5C; or (F) TSG101; or with the non-targeting siRNA transfection control (NT). siRNAs were transfected into HMVEC-L at the concentration of 100 nM for 48 h. Cells were then infected with ANDV (MOI = 0.5) for 24 h or 48 h. Western blots were performed post infection to examine viral Gc and N protein expression. β-actin was probed as the loading control.