Suppression of Protective Responses upon Activation of L-Type Voltage Gated Calcium Channel in Macrophages during Mycobacterium bovis BCG Infection
Fig 2
Activation of VGCC along with M. bovis BCG infection attenuates ROS in mouse and human macrophages.
For Panel A, macrophages were derived from bone marrow of female Balb/c mice. For Panel B, PBMCs from healthy volunteers were differentiated into macrophages. Macrophages were either infected with 2 MOI M. bovis BCG or stimulated with 50 nM BAYK8644 or both for 1h and ROS was estimated as described in Fig 1. In both the Panels, thin line represents ROS generation in uninfected or unstimulated or control cells; thick line represents ROS generation in infected or stimulated cells as indicated. Bar chart below each panel represents MFI of the peak at the higher fluorescence in the figure. Data from one of three independent experiments are shown (n = 3). The star above the bar represents the P value between control and the corresponding group of that bar in each panel. The results were analyzed by one way ANOVA followed by Tukey’s post hoc multiple comparison test. * = P ≤ 0.05; ** = P ≤ 0.01; *** = P ≤ 0.001 and **** = P ≤ 0.0001.