Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2,3-Dioxygenase
Fig 9
Endosomal and not RIG-I pathway inhibitors block IFN-β induction and IDO expression in RSV-infected MSCs.
IFN-β levels were examined in infected MSCs treated with the endosomal pathway inhibitor chloroquine, vehicle control (No Tx), or with RIG-I pathway inhibitor BX795 (A). IDO protein expression was measured in cells treated with chloroquine versus BX795 (B), and cells were examined for RSV PFUs (C). Significance between groups determined by one-way ANOVA with Fisher’s LSD test; n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.