Quantitative Expression Analysis in Brassica napus by Northern Blot Analysis and Reverse Transcription-Quantitative PCR in a Complex Experimental Setting
Fig 5
Normalization of CCA1 with ACT2.
Expression analysis for CCA1 was performed in plants treated as previously described in Fig 3 by Northern blot analysis. Results were normalized using ACT2 according to Fig 3. The normalized (n) and non-normalized (nn) data are presented as the relative expression under (A) diurnal conditions over a period of 36 h and (B) free running conditions with continuous light (LL) in comparison to the light dark (LD) conditions. Data are shown as the mean of three technical replicates ± SD. Relative expression calculation was based on band intensity.