Crystal Structure of the Escherichia coli Fic Toxin-Like Protein in Complex with Its Cognate Antitoxin
Fig 1
Analysis of the various EcFicTA complexes in solution.
(a) SEC-MALLS analysis of EcFicTA (green), EcFicTAE28G (pink), EcFicTG55RA (cyan) and EcFicTG55RAE28G (purple) reveals in each case a 1:1 complex in solution at an eluting concentration of 15 μM. (b) Differential scanning fluorimetry (DSF) analysis of EcFicTA variants. Melting curves (continuous lines) and their first derivatives (broken lines) are shown. Complexes with the E28G mutation in the antitoxin show decreased stability compared to the wild-type complex, whereas the G55R mutation does not affect the thermal stability of the complex.