Cloning and Characterization of Novel Testis-Specific Diacylglycerol Kinase η Splice Variants 3 and 4
Fig 5
Catalytic activities of DGKη3 and η4.
(A) Expression of AcGFP alone, AcGFP-DGKη1, η3 and η4 in COS-7 cells. COS-7 cells were transfected with pAcGFP vector alone, pAcGFP-DGKη1, η3 or η4. The cell lysates (12 μg of protein) were analyzed by Western blotting using anti-GFP antibody. (B) The relative activities of DGKη3 and η4 compared to DGKη1. The cell lysates (5 μg of protein/sample) were assayed for DGK activity (triplicate determinations). The background activities were subtracted and then the values were normalized for DGK expression levels visualized by Western blotting. The results are presented as the percentage of the value of DGKη1 and the mean ± S.D. of the values obtained in three separate experiments.