Suppression of Vimentin Phosphorylation by the Avian Reovirus p17 through Inhibition of CDK1 and Plk1 Impacting the G2/M Phase of the Cell Cycle
Fig 6
ARV infection and ARV p17 transfection inhibits nucleoporin Tpr and activation of p53 and p21.
The levels of nucleoporin Tpr and the levels of p-p53, p-p21, and p-vimentin (Ser 56) in both ARV-infected (A) and p17-transfected Vero cells were examined. Vero cells were either mock-infected or ARV-infected with a MOI of 5 at the indicated time points. Phosphorylation and protein levels were analyzed by Western blot assays with the respective antibodies. The protein levels were normalized to that for β-actin. The fold activation and inactivation indicated below each lane were normalized against the values at 0 h. The levels of the indicated proteins at 0 h were considered 1-fold. The uncropped blots with molecular weights are shown in S11 Fig.