Cardiopulmonary Bypass Down-Regulates NOD Signaling and Inflammatory Response in Children with Congenital Heart Disease
Fig 5
CPB down-regulates expression of the phosphorylated RIP2.
Heparinized whole blood samples were collected from pediatric CHD patients before CPB (Tc), immediately after CPB (T0), at day 1 (T1), day 3 (T3), and day 7 (T7) after CPB for isolation of PBMCs. PBMCs were stimulated with PBS as the control, the NOD1 agonist Tri-DAP (100 ng/ml), and the NOD2 agonist MDP (100 ng/ml) for 30 min. Cytoplasmic proteins were extracted and subjected to Western blot analysis for RIP2 protein expression. Data shown represent one experiment from a total of three separate experiments. P-RIP2 = phosphorylated RIP2. Density ratio of P-RIP2/RIP2 was quantified by densitometry analysis (n = 3). *p<0.05, **p<0.01 compared with Tc.