Decitabine Treatment of Glioma-Initiating Cells Enhances Immune Recognition and Killing
Fig 1
Decitabine has time-dependent cytotoxic effects and is cytostatic for GL261 glioma cells.
(A) GL261-OVA GDC (GDCs) and GL261-OVA GIC (GICs) proliferation measured by CFSE dilution. Cells were CFSE-labelled, plated at the indicated DAC concentrations, and analysed 48h later. Proportions of proliferating GDC and GIC cells, normalised to vehicle controls are shown. *p<0.05;**p<0.01;***p<0.001. One-way Anova test (Dunnet’s multiple comparison test), n = 3. (B) GL261-OVA GDC (GDCs) and GL261-OVA GIC (GICs) percentage cell death measured by LIVE/DEAD fixable yellow dead cell stain after 48h DAC treatment (10 μM) at the indicated timepoints: cell death was measured at time 0 (0h), 24h and 48h after DAC washout (24h and 48h). In the bottom panel, DAC-induced cell death normalised to control is shown. Error bars represent SD. *p<0.05;**p<0.01;***p<0.001. Paired t-test performed (n = 3).