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A Genome-Wide siRNA Screen Implicates Spire1/2 in SipA-Driven Salmonella Typhimurium Host Cell Invasion

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(A) Quality control 1 of the genome wide screen. Cell numbers of chosen controls throughout the whole genome wide screen. (B) Quality control 2 of the genome wide screen. Infection index of chosen controls throughout the whole genome wide screen. Red bars = median of all data points. (C-G) Results of the genome-wide screen are functionally grouped. Color of data points indicates the type of experiment and type of RNAi used. QU = Qiagen unpooled siRNA in HeLa Kyoto cells (blue; original screen). Additional data for knockdown of the presented genes are indicated in green or black: AU = Ambion unpooled siRNA in HeLa CCL-2 cells (green). SE = Sigma esiRNA in HeLa CCL-2 cells (black). Bars represent median of all data points. Asterisks after gene names indicate hit for genome wide QU screen. Panels show proteins indicated in ARP2/3 complex and regulation (C), WAVE, WASH, WASP and interacting proteins (D), RhoGTPases (E), additional actin interacting proteins and regulators (F) and formins (G). (H) KEGG pathway mapping of decreasing hits from the Qiagen unpooled S. TmSipA siRNA screen. Hits reducing the fraction of infected cells below -0.5 median z-score infection index were mapped on all KEGG pathways. KEGG pathway for regulation of actin cytoskeleton is shown. Color code: Strength of the observed phenotype (median z-score). White fields: no hits or no data available from the genome-wide screen. Grey fields were not included in the library or had to be excluded due to low cell number. Adapted from Kreibich et al., 2015.

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doi: https://doi.org/10.1371/journal.pone.0161965.g002