Novel NAD+-Farnesal Dehydrogenase from Polygonum minus Leaves. Purification and Characterization of Enzyme in Juvenile Hormone III Biosynthetic Pathway in Plant
Fig 5
Effect of temperature towards activity of farnesal dehydrogenase.
(×) Temperature optimum, (●) residual activity after heat treatment. The optimal temperature was determined by performing the standard enzyme assay as described in “Materials and Methods,” except that the reaction temperature was varied. The effect of temperature on residual activity of the enzyme was determined by incubating the purified enzymes at a temperature in the range of 20–70°C for 10 min at pH 7.5 (100 mM tricine-NaOH containing 2.5 mM 2-ME). Relative activity values for temperature are indicated as mean values (n = 3).