Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies
Fig 2
A. SDS-PAGE of Ni-NTA affinity purification of recombinant TYR456 and 6xHis-tag cleavage. M, molecular weight marker; MD, concentrated medium; FT, flow-through fraction; W, wash fraction; E, elution fraction; TC, TEV-protease cleaved fraction. B. Ion exchange chromatogram of TYR456, upon applying a NaCl gradient from 50 mM to 500 mM. Two elution peaks are observed (A and B), both corresponding to recombinant tyrosinase samples. Peak B eluting at ~150 mM NaCl was selected for further analysis.