Small GTPases Rab8a and Rab11a Are Dispensable for Rhodopsin Transport in Mouse Photoreceptors
Fig 7
Rhodopsin localizes normally in adult Rab8a KO mice injected with Rab8b(T22N) AAV.
(A) Alignment of Rab8a and Rab8b amino acid sequences. Exon 1 contains the GTP binding domain. Exon 2 is deleted in the germline and conditional knockouts. T22, preventing GTP binding when mutated, is shown. (B) Genotyping confirms deletion of exon2 in retRab8a-/- retinas but not tails. retRab8a+/- tissues were used as controls. (C) Immunofluorescence showed that Rab8a is mainly localized as punctate signal in IS and OPL in WT but absent in retRab8a-/- photoreceptor cells. (D) Rab8b(T22N) is mainly localized at IS and OPL. (E) Rhodopsin, GC1, CNGA1/A3 and ribeye are localized correctly in 4M-old retRab8a-/- and retRab8a+/- animals injected with dnRab8b (Rab8b(T22N)) AAV. Scale bars, 20 μm (C and D) and 30 μm (E).