Epitope Mapping of a Monoclonal Antibody Directed against Neisserial Heparin Binding Antigen Using Next Generation Sequencing of Antigen-Specific Libraries
Fig 4
Immunoreactivity of mAb 31E10/E7 to a panel of recombinant NHBA-NUbp fragments spotted in a microarray.
Each horizontal bar represents a protein or protein fragment in the microarray aligned along the NHBA-NUbp sequence and color-coded from light grey to dark red according to mean fluorescence intensity (MFI) values, as shown in the vertical bar. The blue box indicates the sequence shared by all positive fragments (A87-S118). Shown are MFI values from one experiment representative of three, each performed using 8 replicates per protein. Raw data from all experiments are available under accession number GSE81379 at the National Center for Biotechnology Information’s Gene Expression Omnibus database.