Cystathionine-Gamma-Lyase Gene Deletion Protects Mice against Inflammation and Liver Sieve Injury following Polymicrobial Sepsis
Fig 5
Effect of CSE Gene Deletion on Liver Pro-inflammatory Cytokine and Chemokine Levels Following CLP Induced Sepsis.
(A) Liver TNF-α, (B) Liver IL-6, (C) Liver IL-1β, (D) Liver MCP-1 and (E) Liver MIP-2α. CLP induced sepsis significantly increased the liver cytokines TNF-α (P<0.05), IL-6 (P<0.05) and IL-1β (P<0.0001) and the chemokines MCP-1 (P<0.0001) and MIP-2α (P<0.0001) in WT mice compared to sham operation controls. Knockdown of the CSE gene protects mice against liver injury by reducing pro-inflammatory TNF-α (P<0.01), IL-6 (P<0.01), IL-1β (P<0.05), MCP-1 (P<0.01) and MIP-2α (P<0.001) following CLP induced sepsis compared to WT sepsis mice. Results were expressed in ng/mg of protein. Data represent the mean±standard deviation (n = 8). Data were analysed for Gaussian or Normal distribution using Shapiro-Wilk test. One-way ANOVA with post hoc Tukey’s test was performed to compare multiple groups. Statistical significance was assigned as *P<0.05; **P<0.01: ***P<0.001; and ****P<0.0001.