Induction of Suppressor Cells and Increased Tumor Growth following Chronic Psychosocial Stress in Male Mice
Fig 1
Effect of CSC on spleen cells.
Splenocytes from CSC (black bars) and SHC mice (white bars) were isolated and analyzed by flow cytometry (total splenocytes SHC, n = 7: 4.2±1.7x107; CSC, n = 8: 9.8±2.4x107). The percentage of splenic B (SHC: 1.9±0.8x107; CSC: 3.2±0.8x107) and T cells (SHC: 1.6±0.3x107; CSC: 2.6±0.6x107) (A) and Treg cells (SHC: 2.0±0.4x106; CSC: 2.6±0.6x106) (B) were quantified after 19d of SHC/CSC. Splenic CD11b+ cells (C) and CD11b+ cells differentiated by their Gr1 expression level were analyzed after 48h and 19d of CSC (SHC: n = 8, CSC: n = 8) (D). Immunohistochemical staining of spleen sections from mice after 19d of CSC/SHC for CD11b+ (blue), Gr1+ (green), and B220+ (red) cells was performed (E). Splenocytes from individual mice after 19d CSC/SHC were stimulated with LPS followed by determination of TNF, IL-6, and IL-10 levels in the supernatants (SHC: n = 4, CSC: n = 4) (F). *p < 0.05; **p < 0.01; ***p < 0.001 (Student´s t-test). These experiments were performed at least 3 times.