miR-30e Blocks Autophagy and Acts Synergistically with Proanthocyanidin for Inhibition of AVEN and BIRC6 to Increase Apoptosis in Glioblastoma Stem Cells and Glioblastoma SNB19 Cells
Fig 6
Target prediction for miR-30e and also RT-PCR and Western blotting to examine levels of expression of AVEN and BIRC6 in GSC and SNB19 cells.
(A) Bioinformatics analysis showing AVEN and BIRC6 as potential targets for miR-30e. (B) RT-PCR analysis and band density quantification to determine relative mRNA expression of AVEN and BIRC6 in GSC and SNB19 cells. Treatments: untreated control (CTL); 2 mM SS for 24 h + 50 nM miR-30e for 12 h; 2 mM SS for 24 h + 150 μM PAC for 24 h; and 2 mM SS for 24 h + 50 nM miR-30e for 12 h + 150 μM PAC for 24 h. (C) Western blotting for examining protein expression of AVEN and BIRC6 in GSC and SNB19 cells. Treatments: untreated control (CTL); 2 mM SS for 24 h + 50 nM miR-30e for 12 h; 2 mM SS for 24 h + 150 μM PAC for 24 h; and 2 mM SS for 24 h + 50 nM miR-30e for 12 h + 150 μM PAC for 24 h. Western blots to show inhibition of protein expression of AVEN and BIRC6.